| 质粒名称: | U6 pShuttle |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13428 |
| 存储实验室: | Ronald Kahn |
|
载体骨架基本信息 |
|
| 载体名称: | pShuttle |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 人U6启动子插入pShuttle |
| 载体类型: | 哺乳细胞表达,腺病毒 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | LKO.1 5' |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | U6 pShuttle was a gift from Ronald Kahn (Addgene plasmid # 13428 ; http://n2t.net/addgene:13428 ; RRID:Addgene_13428) |
买家导航
U6pShuttle载体基本信息
U6pShuttle载体图谱质粒图谱和多克隆位点信息
U6pShuttle载体简介
U6pShuttle载体序列
LOCUS U6 pShuttle 6967 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS U6 pShuttle
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6967)
TITLE pShuttle vectors
REFERENCE 2 (bases 1 to 6967)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6967
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..103
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
promoter 390..630
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 390..410
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 561..580
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
repeat_region 3926..4028
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
primer_bind complement(4117..4134)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4288..4876)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4368..4387)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 5708..6502
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5762..5781)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6372..6391
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
rep_origin complement(6550..6930)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6612..6633)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6824..6843
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcggccgcct
361 cgacgcgtgg atccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
421 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
481 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
541 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
601 tcttggcttt atatatcttg tggaaaggac gaaacaccgt gctcgcttcg gcagcacata
661 tactagtcga cagtactggt acctctagag cggacttcgg tccgcttttt actaggacct
721 gcaggcatgc aagcttggga gatctgggcg tggttaaggg tgggaaagaa tatataaggt
781 gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagcaccaac
841 tcgtttgatg gaagcattgt gagctcatat ttgacaacgc gcatgccccc atgggccggg
901 gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct
961 actaccttga cctacgagac cgtgtctgga acgccgttgg agactgcagc ctccgccgcc
1021 gcttcagccg ctgcagccac cgcccgcggg attgtgactg actttgcttt cctgagcccg
1081 cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac ggctcttttg
1141 gcacaattgg attctttgac ccgggaactt aatgtcgttt ctcagcagct gttggatctg
1201 cgccagcagg tttctgccct gaaggcttcc tcccctccca atgcggttta aaacataaat
1261 aaaaaaccag actctgtttg gatttggatc aagcaagtgt cttgctgtct ttatttaggg
1321 gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt cgttgagggt cctgtgtatt
1381 ttttccagga cgtggtaaag gtgactctgg atgttcagat acatgggcat aagcccgtct
1441 ctggggtgga ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc
1501 cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt
1561 gccaggggca ggcccttggt gtaagtgttt acaaagcggt taagctggga tgggtgcata
1621 cgtggggata tgagatgcat cttggactgt atttttaggt tggctatgtt cccagccata
1681 tccctccggg gattcatgtt gtgcagaacc accagcacag tgtatccggt gcacttggga
1741 aatttgtcat gtagcttaga aggaaatgcg tggaagaact tggagacgcc cttgtgacct
1801 ccaagatttt ccatgcattc gtccataatg atggcaatgg gcccacgggc ggcggcctgg
1861 gcgaagatat ttctgggatc actaacgtca tagttgtgtt ccaggatgag atcgtcatag
1921 gccattttta caaagcgcgg gcggagggtg ccagactgcg gtataatggt tccatccggc
1981 ccaggggcgt agttaccctc acagatttgc atttcccacg ctttgagttc agatgggggg
2041 atcatgtcta cctgcggggc gatgaagaaa acggtttccg gggtagggga gatcagctgg
2101 gaagaaagca ggttcctgag cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca
2161 cctattaccg gctgcaactg gtagttaaga gagctgcagc tgccgtcatc cctgagcagg
2221 ggggccactt cgttaagcat gtccctgact cgcatgtttt ccctgaccaa atccgccaga
2281 aggcgctcgc cgcccagcga tagcagttct tgcaaggaag caaagttttt caacggtttg
2341 agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa gcagttccag gcggtcccac
2401 agctcggtca cctgctctac ggcatctcga tccagcatat ctcctcgttt cgcgggttgg
2461 ggcggctttc gctgtacggc agtagtcggt gctcgtccag acgggccagg gtcatgtctt
2521 tccacgggcg cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg
2581 gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt
2641 cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc agcccctccg
2701 cggcgtggcc cttggcgcgc agcttgccct tggaggaggc gccgcacgag gggcagtgca
2761 gacttttgag ggcgtagagc ttgggcgcga gaaataccga ttccggggag taggcatccg
2821 cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg
2881 ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt cttacctctg gtttccatga
2941 gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca gacttgagag
3001 ggagtttaaa cgaattcaat agcttgttgc atgggcggcg atataaaatg caaggtgctg
3061 ctcaaaaaat caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc
3121 agataaaggc aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac
3181 atgtctgcgg gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag
3241 aagcctgtct tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc
3301 cggcgtgacc gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg
3361 tcatgtccgg agtcataatg taagactcgg taaacacatc aggttgattc acatcggtca
3421 gtgctaaaaa gcgaccgaaa tagcccgggg gaatacatac ccgcaggcgt agagacaaca
3481 ttacagcccc cataggaggt ataacaaaat taataggaga gaaaaacaca taaacacctg
3541 aaaaaccctc ctgcctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt
3601 ccacagcggc agccataaca gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac
3661 accactcgac acggcaccag ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc
3721 gagtatatat aggactaaaa aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa
3781 accgcacgcg aacctacgcc cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg
3841 tcacttccgt tttcccacgt tacgtcactt cccattttaa gaaaactaca attcccaaca
3901 catacaagtt actccgccct aaaacctacg tcacccgccc cgttcccacg ccccgcgcca
3961 cgtcacaaac tccaccccct cattatcata ttggcttcaa tccaaaataa ggtatattat
4021 tgatgatgtt aattaacatg catggatcca tatgcggtgt gaaataccgc acagatgcgt
4081 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5341 gcgcaacgtt gttgccattg ctgcagccat gagattatca aaaaggatct tcacctagat
5401 ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
5461 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
5521 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
5581 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
5641 gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc
5701 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
5761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
5821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
5881 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
5941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
6001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
6061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
6121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
6181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
6241 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
6301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
6361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
6421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
6481 ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta
6541 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
6601 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
6661 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
6721 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
6781 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
6841 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
6901 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
6961 taattaa
//
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